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Workplace Drug and Alcohol Testing Programs For Transportation Industry

The Department of Transportation's (DOT) rule, 49 CFR Part 40, describes required procedures for conducting workplace drug and alcohol testing for the Federally regulated transportation industry.

View the current 49 CFR Part 40 in its entirety 40.1

To view 49 CFR Part 40 by section and related Q&As, click on the relevant subparts below.

Subpart A
Administrative Provisions

Subpart B
Employer Responsibilities

Subpart C
Urine Collection Personnel

Subpart D
Collection Sites, Forms, Equipment and Supplies Used in DOT Urine Collections

Subpart E
Urine Specimen Collections

Subpart F
Drug Testing Laboratories

Subpart G
Medical Review Officers and the Verification Process

Subpart H
Split Specimen Tests

Subpart I
Problems in Drug Tests

Subpart J
Alcohol Testing Personnel

Subpart K
Testing Sites, Forms, Equipment and Supplies Used in Alcohol Testing

Subpart L
Alcohol Screening Tests

Subpart M
Alcohol Confirmation Tests

Subpart N
Problems in Alcohol Testing

Subpart O
Substance Abuse Professionals and the Return-to-Duty Process

Subpart P
Confidentiality and Release of Information

Subpart Q
Roles and Responsibilities of Service Agents

Subpart R
Public Interest Exclusions

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